Bioinformatics Solutions

Bioinformatics is used in a vast array of analyses of gene variation and expression and more generally in understanding evolutionary aspects of molecular biology. Our solutions help you with your bioinformatic analysis of next-generation and genotyping data. We offer the following bioinformatics services:

Data Analysis of Sequencing Runs

Comprehensive method to analyse the entire genome of an individual. Proven to be essential and invaluable for the identification of gene functions and their involvement in disease.

Applications:

Helps in surveying exonic regions that are poorly captured, and be used to detect structural variants, especially in the case of newborn and pediatric diseases.
Helps pharmacologists to tailor disease treatments to an individual’s genetic makeup.
Major applications in Mendelian diseases, complex diseases, and cancers in medical genetics.
Enables the identification of additional classes of mutations like large structural rearrangements, balanced translocations, uniparental isodisomy, and mosaicism
Helps in providing information concerning the genetic basis of phenotypic characteristics, including antibiotic susceptibility and virulence

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Whole transcriptome sequencing also known as Total RNA-Seq is the sequencing of coding and non-coding regions of the RNA. The aim of whole transcriptome sequencing is to get all the RNA transcripts of an organism. Total RNA-Seq is vital for qualitative and quantitative levels of RNA in respective biological samples at given time spans.

Applications:

Deciphers genome structure and function, identifying genetic networks underlying cellular, physiological, biochemical and biological systems and establishing molecular biomarkers that respond to diseases, pathogens and environmental challenges.
Quantifies gene expression heterogeneity in cells, tissues, organs and even a whole body.
Characterizes novel instances of lincRNAs and may contribute to important areas of cancer research.

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It is extensively used to selectively capture and sequence protein-coding regions of the genome. The protein-coding regions account for only 2% of the whole genome. WES covers more than 85% of this region encompassing disease-causing and genetically influencing variants. The mutations detected using WES have applications in population genetics, cancer studies and secondary or incidental findings.

Applications:

Provides a novel approach to personalized medicine
Paved way for predictive and prognostic testing, prenatal genetics, multifactorial diseases, pharmacogenetics, and targeted treatments for cancer
Widely used to study natural evolution in plants, host-pathogen interactions and exonic aid in the improvement of crops

Analysis of small RNA genome (fragment or full spectrum) of biological systems. An RNA-Seq protocol can isolate, sequence and analyse small RNAs such as miRNAs. The ever-developing field of miRNA sequencing requires standardized and effective bioinformatics protocols to analyse the data. Micro-RNAs are short, single-stranded RNAs that are used in post-transcriptional gene expression regulation or translation repression.

Applications:

Tissue-specific differential expression analysis of small RNAs
Discovery of novel biomarkers (miRNA discovery) for cancer, and other diseases

Microbes are present almost everywhere. They play a vital role in cycling carbon, release important compounds, and may be associated with infectious diseases. Metagenomics presents a powerful tool to study prokaryotes and viruses in the environment via the analysis of their DNA obtained directly from environmental samples. It can identify the microbial species present and provide insight into the functional roles and metabolic activities of the microorganisms. The coupling of this method with function-based activity is a powerful technique for the discovery of new functional genes from uncultured microbes.

Mitochondrial disorders are heterogeneous in nature which becomes a problem while studying the mutation. Next-generation sequencing (NGS) addresses these challenges. NGS has enabled and enhanced the diagnosis of mitochondrial disorders. NGS has enabled a systematic analysis of whole mitochondrial DNA and better detection sensitivity. The sequencing data so obtained require to be analysed by bioinformatics tools, these tools allow the detection and quantification of mtDNA variants.

The 16S rRNA amplicon sequencing data analysis technique amplifies small fragments of hypervariable regions of the 16S rRNA gene. 16S rRNA gene encodes the RNA component of the small subunit of ribosomes. They contain regions that are less evolutionarily constrained and whose sequences indicate their phylogeny.

Applications:

Identify and compare bacteria or fungi present within a given sample.
Useful in studying phylogeny and taxonomy from complex microbiomes or environments.
Proven to be an efficient and cost-effective strategy for microbiome analysis and even allows for the imputation of functional content based on taxon abundances.

Genotyping By Array

Also known as DNA genotyping using microarray, is a popular technique for detecting polymorphism in populations. It is a feasible method for detecting single nucleotide polymorphisms (SNPs). This is a cost-efficient method for detecting variants as compared to next-generation sequencing. Microarrays are capable of giving deep insights into variants associated with diseased conditions at a molecular level.

Applications:

Provide economical solutions for population-scale genetic studies, variant screening, and precision medicine research
Helps especially in the identification of single-nucleotide polymorphisms (SNPs) and mutations, classification of tumours, identification of target genes of tumour suppressors, identification of cancer biomarkers, identification of genes associated with chemoresistance, and drug discovery

Our Pipeline / Workflow automation service can help you to make your complex manual pipeline into a one-click robust and automatic solution for better and uniform outcomes.

This solution must be opted for when you have:

To handle complex analysis pipeline
To process ample amounts of samples
To perform uniform analysis of all your samples

Workflow Automation

Database Development with customised GUI

Our Database Development with Customized GUI service can help you to make a robust and easy-to-use graphics-based database to explore and meta-analysis to get new findings out of all your projects

This solution must be opted for when you:

Have multiple projects at-hand
Struggle to find a specific file from the specific project every time you need it
Want to do meta-analysis or statistics on previous studies
Want to correlate your current study with previous ones